Enhanced biological activity of Candida rugosa lipase via immobilization on magnetic nanoparticles
نویسندگان
چکیده
منابع مشابه
Optimization of Candida rugosa lipase immobilization parameters on magnetic silica aerogel using adsorption method
Magnetic silica aerogel in hydrophobic and hydrophilic forms were used as support to immobilize Candida rugosa lipase by adsorption method. Response surface methodology (RSM) was employed to study the effects of the three most important immobilization parameters, namely enzyme/support ratio (0.3-0.5, w/w), immobilization time (60-120 min) and alcohol percentage (20-40, %v/v) on the specific act...
متن کاملOptimization of Biodiesel Production Using Immobilized Candida Rugosa Lipase on Magnetic Fe3O4-Silica Aerogel
Hydrophobic magnetic silica aerogel was used as a support to immobilize Candida rugosa lipase by adsorption method. Physical and chemical properties of the support and immobilized lipase were determined by Field Emission Scanning Electron Microscope (FESEM), Brunauer–Emmett–Teller (BET) analysis and Fourier Transform InfraRed (FT-IR) spectroscopy and the results showed that the lipase was s...
متن کاملImmobilization of Candida antarctica lipase B on Polystyrene Nanoparticles.
Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal-B) and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized Cal-B was studied. The pH of the buffer solution has no influence on enzyme loading, while ...
متن کاملImmobilization of lipase from Candida rugosa on synthetic polymer beads for use in the synthesis of fatty esters ABSTRACT Lipase from Candida rugosa
Lipase from Candida rugosa was immobilized on three different supports, i.e. Amberlite XAD7, poly(methylmethacrylate) (PMMA) and celite. With the conditions tested, maximum adsorption can be achieved after 30 min. The activities of the immobilized lipases were determined by the esterification reaction of oleic acid and butanol. The immobilized lipases were found to be very effective in the este...
متن کاملImmobilization of Candida rugosa lipase on MCM-41 for the transesterification of cotton seed oil.
Present study demonstrated the preparation of MCM-41 as a support for the immobilization of Candida rugosa lipase by the physical adsorption technique. The lipase immobilized MCM-41 has been characterized by scanning electron microscopic and FTIR techniques. At pH 6, maximum lipase immobilization (250 mg/g) on MCM support has been observed and the immobilized lipase was employed as biocatalyst ...
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ژورنال
عنوان ژورنال: Egyptian Journal of Chemistry
سال: 2020
ISSN: 2357-0245
DOI: 10.21608/ejchem.2020.25662.2512